Journal: Materials Today Bio

Article Title: Cuprorivaite microspheres inhibit cuproptosis and oxidative stress in osteoarthritis via Wnt/β-catenin pathway

doi: 10.1016/j.mtbio.2024.101300

Figure Lengend Snippet: Anti-inflammatory role of Cuprorivaite (CaCuSi 4 O 10 ) microspheres in IL-1β-stimulated chondrocytes. (A) Cell counting kit-8 test for cell proliferation. (B) Fluorescein diacetate for cell viability detection. (C) Cell apoptosis by flow cytometry. (D) ELISA detection for TNF-α, IL-6 and MMP13 in cell supernatant. (E) Western blot detection for TNF-α, IL-6 and MMP13 in cell lysates. (F) RT-qPCR quantification for in TNF-α, IL-6 and MMP13. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: Then, levels of TNF-α, IL-6, and MMP13 in cell supernatant and serum samples were detected by the multi-functional microplate detector, with mouse TNF-α (CSB-E04741m, CUSABIO, Wuhan, China), mouse IL-6 (CSB-E04639m, CUSABIO, Wuhan, China) and mouse MMP13 (CSB-E07413m, CUSABIO, Wuhan, China) kit.

Techniques: Cell Counting, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR

Journal: Materials Today Bio

Article Title: Cuprorivaite microspheres inhibit cuproptosis and oxidative stress in osteoarthritis via Wnt/β-catenin pathway

doi: 10.1016/j.mtbio.2024.101300

Figure Lengend Snippet: Cuprorivaite (CaCuSi 4 O 10 ) microspheres improved IL-1β-induced injury in chondrocytes via inhibiting Wnt/β-catenin pathway. (A) Cell counting kit-8 test for cell viability. (B) Fluorescein diacetate for cell viability detection. (C) Cell apoptosis by flow cytometry. (D) ELISA detection for TNF-α, IL-6 and MMP13 in cell supernatant. (E) Western blot detection for TNF-α, IL-6 and MMP13 in cell lysates. (F) RT-qPCR quantification for extracellular matrix components including collagen II and SOX9. (G) Intracellular copper content. (H) RT-qPCR quantification for cuproptosis biomarkers including ATP7B and FDX1. (I) Western blot detection for ATP7B and FDX1. (J) Oxidative stress detection including MDA, SOD and GSH. (K) RT-qPCR quantification for Wnt1, GSK3β and β-catenin. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: Then, levels of TNF-α, IL-6, and MMP13 in cell supernatant and serum samples were detected by the multi-functional microplate detector, with mouse TNF-α (CSB-E04741m, CUSABIO, Wuhan, China), mouse IL-6 (CSB-E04639m, CUSABIO, Wuhan, China) and mouse MMP13 (CSB-E07413m, CUSABIO, Wuhan, China) kit.

Techniques: Cell Counting, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR

Journal: Materials Today Bio

Article Title: Cuprorivaite microspheres inhibit cuproptosis and oxidative stress in osteoarthritis via Wnt/β-catenin pathway

doi: 10.1016/j.mtbio.2024.101300

Figure Lengend Snippet: Validation of mechanism of Cuprorivaite (CaCuSi 4 O 10 ) microspheres in OA. (A) Hematoxylin and eosin staining. (B) Safranin-O staining/fast green staining. (C) OARSI score. (D) ELISA detection for TNF-α, IL-6 and MMP13 in the serum. (E) Western blot detection for TNF-α, IL-6 and MMP13 in cartilage tissue. (F) RT-qPCR quantification for extracellular matrix components including collagen II and SOX9 in cartilage tissue. (G) Copper content in cartilage tissue. (H) RT-qPCR quantification for cuproptosis biomarkers including ATP7B and FDX1 in cartilage tissue. (I) Western blot detection for ATP7B and FDX1 in cartilage tissue. (J) Representative images of FDX1 expression in cartilage tissue detected by immunohistochemistry. (K) Oxidative stress detection including MDA, SOD and GSH in cartilage tissue. (L) RT-qPCR quantification for Wnt1, GSK3β and β-catenin in cartilage tissue. ∗∗∗ P < 0.001.

Article Snippet: Then, levels of TNF-α, IL-6, and MMP13 in cell supernatant and serum samples were detected by the multi-functional microplate detector, with mouse TNF-α (CSB-E04741m, CUSABIO, Wuhan, China), mouse IL-6 (CSB-E04639m, CUSABIO, Wuhan, China) and mouse MMP13 (CSB-E07413m, CUSABIO, Wuhan, China) kit.

Techniques: Biomarker Discovery, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR, Expressing, Immunohistochemistry

Journal: Frontiers in Pharmacology

Article Title: Modified Si Miao Powder granules alleviates osteoarthritis progression by regulating M1/M2 polarization of macrophage through NF-κB signaling pathway

doi: 10.3389/fphar.2024.1361561

Figure Lengend Snippet: MSMP affected cartilage matrix metabolism in OA mice. (A) Immunohistochemical staining of Col2a1. (B) Statistical analysis of the positive expression for Col2a1. (C) Immunohistochemical staining of Mmp13. (D) Statistical analysis of the positive expression for Mmp13. Scale bar represents 100 μm (Date are mean ± SD, #### p < 0.0001, versus Sham group; * p < 0.05, ** p < 0.01, **** p < 0.0001, versus OA group).

Article Snippet: The reagents in the current study were as follows: Lipopolysaccharide (Sigma), p65 Antibody (Abways, CY5034), p-p65 Antibody (Abways, CY6372), β-actin Antibody (Boster, BM3873), Col2a1 Antibody (Boster, BA0533), Mmp13 Antibody (Boster, BA2204), iNOS Antibody (Boster, BA0362), Arg1 Antibody (Servicebio, GB11285-100), IL-6 ELISA Kit (Boster), TNF-α ELISA Kit (Boster), IL-10 ELISA Kit (RuiXin Bio), HE Staining Kit (Solarbio), Senna O Solid Green Staining Kit (Solarbio) Animal Surgical Instruments, Absorbable Surgical Suture (6-0), Gavage Needle (12 gauge), Tissue Decalcification Solution (Bosterbio), Tissue Fixation Solution (Bosterbio), and Sodium Pentobarbital.

Techniques: Immunohistochemical staining, Staining, Expressing